Publications of Manfred T. Reetz

Li, A.; Acevedo-Rocha, C. G.; Reetz, M. T. Boosting the Efficiency of Site-Saturation Mutagenesis for a Difficult-to-Randomize Gene by a Two-Step PCR Strategy. Applied Microbiology and Biotechnology 2018, 102, 6095–6103.

Acevedo-Rocha, C. G.; Gamble, C. G.; Lonsdale, R.; Li, A.; Nett, N.; Hoebenreich, S.; Lingnau, J. B.; Wirtz, C.; Fares, C.; Hinrichs, H.; Deege, A.; Mulholland, A. J.; Nov, Y.; Leys, D.; McLean, K. J.; Munro, A. W.; Reetz, M. T. P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping. ACS Catalysis 2018, 8, 3395–3410.

Li, G.; Wang, J.; Reetz, M. T. Biocatalysts for the Pharmaceutical Industry Created by Structure-Guided Directed Evolution of Stereoselective Enzymes. Bioorganic & Medicinal Chemistry 2018, 26, 1241–1251.

Li, A.; Acevedo-Rocha, C. G.; Sun, Z.; Cox, T.; Xu, J. L.; Reetz, M. T. Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction. Chembiochem 2018, 19, 221–228.

Qu, G.; Lonsdale, R.; Yao, P.; Li, G.; Liu, B.; Reetz, M. T.; Sun, Z. Methodology Development in Directed Evolution: Exploring Options when Applying Triple-Code Saturation Mutagenesis. Chembiochem 2018, 19, 239–246.

Reetz, M. T. Combinatorial Libraries Reloaded^[1]. Israel Journal of Chemistry 2018, 58, 52–60.

Sun, Z.; Wu, L.; Bocola, M.; Chan, H. C. S.; Lonsdale, R.; Kong, X.-D.; Yuan, S.; Zhou, J.; Reetz, M. T. Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations. Journal of the American Chemical Society 2018, 140, 310–318.

Li, G.; Fürst, M. J. L. J.; Mansouri, H. R.; Ressmann, A. K.; Ilie, A.; Rudroff, F.; Mihovilovic, M. D.; Fraaije, M. W.; Reetz, M. T. Manipulating the Stereoselectivity of the Thermostable Baeyer–Villiger Monooxygenase TmCHMO by Directed Evolution. Organic & Biomolecular Chemistry 2017, 15, 9824–9829.

Li, G.; Maria-Solano, M. A.; Romero-Rivera, A.; Osuna, S.; Reetz, M. T. Inducing High Activity of a Thermophilic Enzyme at Ambient Temperatures by Directed Evolution. Chemical Communications 2017, 53, 9454–9457.

Wang, J.; Ilie, A.; Yuan, S.; Reetz, M. T. Investigating Substrate Scope and Enantioselectivity of a Defluorinase by a Stereochemical Probe. Journal of the American Chemical Society 2017, 139, 11241–11247.

Wang, J.; Ilie, A.; Reetz, M. T. Chemo- and Stereoselective Cytochrome P450-BM3 Catalyzed Sulfoxidation of 1-Thiochroman-4-Ones. Advanced Synthesis & Catalysis 2017, 359, 2056–2060.

Li, G.; Yao, P.; Gong, R.; Li, J.; Liu, P.; Lonsdale, R.; Wu, Q.; Lin, J.; Zhu, D.; Reetz, M. T. Correction: Simultaneous Engineering of an Enzyme’s Entrance Tunnel and Active Site: the Case of Monoamine Oxidase MAO-N. Chemical Science 2017, 8, 4666–4666.

Acevedo, J. P.; Reetz, M. T.; Asenjo, J. A.; Parra, L. P. One-Step Combined Focused EpPCR and Saturation Mutagenesis for Thermostability Evolution of a New Cold-Active Xylanase. Enzyme and Microbial Technology 2017, 100, 60–70.

Li, G.; Yao, P.; Gong, R.; Li, J.; Liu, P.; Lonsdale, R.; Wu, Q.; Lin, J.; Zhu, D.; Reetz, M. T. Simultaneous Engineering of an Enzyme's Entrance Tunnel and Active Site: the Case of Monoamine Oxidase MAO-N. Chemical Science 2017, 8, 4093–4099.

Wang, J.; Li, G.; Reetz, M. T. Enzymatic Site-Selectivity Enabled by Structure-Guided Directed Evolution. Chemical Communications 2017, 53, 3916–3928 .

Li, A.; Wang, B.; Ilie, A.; Dubey, K. D.; Bange, G.; Korendovych, I. V.; Shaik, S.; Reetz, M. T. A Redox-Mediated Kemp Eliminase. Nature Communications 2017, 8, 14876.

Qiao, Y.; Theyssen, N.; Eifert , T.; Liauw, M. A.; Francio, G.; Schenk , K.; Leitner, W.; Reetz, M. T. Concerning the Role of Supercritical Carbon Dioxide in SN₁ Reactions. Chemistry – A European Journal 2017, 23, 3898–3902.

Ilie, A.; Roiban, G.-D.; Reetz, M. T. Di–Tert–Butyl N,N-Diethylphosphoramidite as an Air Stable Ligand for Suzuki-Miyaura and Buchwald-Hartwig Reactions. ChemistrySelect 2017, 2, 1392–1397.

Luo, X.-J.; Zhao, J.; Li, C.-X.; Bai, Y.-P.; Reetz, M. T.; Yu, H.-L.; Xu, J.-H. Combinatorial Evolution of Phosphotriesterase Toward a Robust Malathion Degrader by Hierarchical Iteration Mutagenesis. Biotechnology and Bioengineering 2016, 113, 2350–2357.

Sun, Z.; Lonsdale, R.; Li, G.; Reetz, M. T. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single- Versus Double-Code Saturation Mutagenesis. Chembiochem 2016, 17, 1865–1872.