Publications of Yosephine Gumulya

Gumulya, Y.; Sanchis-Martinez, J.; Reetz, M. T. Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local MinimaYosephiné. ChemBioChem: A European Journal of Chemical Biology 2012, 13, 1060–1066.

Gumulya, Y.; Reetz, M. T. Enhancing the Thermal Robustness of an Enzyme by Directed Evolution: Least Favorable Starting Points and Inferior Mutants Can Map Superior Evolutionary Pathways. ChemBioChem 2011, 12, 2502–2510.

Fernández, L.; Jiao, N.; Soni, P.; Gumulya, Y.; De Oliveira, L. G.; Reetz, M. T. An Efficient Method for Mutant Library Creation in Pichia Pastoris Useful in Directed Evolution. Biocatalysis and Biotransformation 2010, 28, 122–129.

Reetz, M. T.; Prasad, S.; Carballeira, J. D.; Gumulya, Y.; Bocola, M. Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods. Journal of the American Chemical Society 2010, 132, 9144–9152.

Reetz, M. T.; Soni, P.; Fernández, L.; Gumulya, Y.; Carballeira, J. D. Increasing the Stability of an Enzyme Toward Hostile Organic Solvents by Directed Evolution Based on Iterative Saturation Mutagenesis Using the B-FIT Method. Chemical Communications (Cambridge) 2010, 46, 8657–8658.

Sanchis, J.; Fernández, L.; Carballeira, J. D.; Drône, J.; Gumulya, Y.; Höbenreich, H.; Kahakeaw, D.; Kille, S.; Lohmer, R.; Peyralans, J. J.-P.; Podtetenieff, J.; Prasad, S.; Soni, P.; Taglieber, A.; Wu, S.; Zilly, F. E.; Reetz, M. T. Improved PCR Method for the Creation of Saturation Mutagenesis Libraries in Directed Evolution: Application to Difficult-to-Amplify Templates. Applied Microbiology and Biotechnology 2008, 81, 387–397.

Gumulya, Y. Exploration of Evolutionary Pathways Aspergillus Niger Epoxide Hydrolase. PhD Thesis, Ruhr-Universität Bochum, Bochum, 2010.